(a) Alignment of cat, mouse and human Dkk4 proteins showing conservation of the N‐terminal signal peptide (blue shading) and cleavage site and cysteine‐rich domains CRD1 and CRD2. Animal Genetics published by John Wiley & Sons Ltd on behalf of Stichting International Foundation for Animal Genetics.īioinformatic analysis of DKK4 variants. All coding variants were excluded as Ticked alleles using Strategy B.ĭKK4 Dickkopf Wnt Signaling Pathway Inhibitor 4 Tabby Abyssinian coat pattern.
Bioinformatic and molecular modeling analysis suggests that these changes disrupt a key disulfide bond in the Dkk4 cysteine-rich domain 1 or Dkk4 signal peptide cleavage respectively. For Strategy A, two variants in Dickkopf Wnt Signaling Pathway Inhibitor 4 (DKK4), a p.Cys63Tyr (B1:41621481, c.188G>A) and a less common p.Ala18Val (B1:42620835, c.53C>T) variant are suggested as two alleles influencing the Ticked phenotype. Two strategies were used to find the Ticked allele(s), one considered Cinnamon with the reference allele or heterozygous (Strategy A) and the other considered Cinnamon as having the variant allele or heterozygous (Strategy B). The cat reference genome (Cinnamon, the Abyssinian) has the ticked phenotype and the variant dataset and coat phenotypes from the 99 Lives Cat Genome Consortium (195 cats) were used to identify candidate genes and variants associated with the Ticked locus. The unidentified gene for the Ticked locus on cat chromosome B1 was suggested to control the presence or absence of the ticked pattern (Tabby - Abyssinian (T a a.k.a. The Tabby locus is laeverin (LVRN) and affects the mackerel and blotched patterns. Currently, at least three loci influence cat tabby markings, two of which are designated Tabby and Ticked. classic, blotched) were thought to control these patterns in domestic cats and their breeds. Historically, three autosomal alleles at one locus (Tabby): Abyssinian (T a a.k.a. Tabby patterns of fur coats are defining characteristics in wild and domestic felids. Marie Abitbol, Danielle Aberdein, Paulo C Alves, Asa Ohlsson Andersson, Rebecca R Bellone, Tomas F Bergström, Nuket Bilgen, Adam R Boyko, Jeffrey A Brockman, Margret L Casal, Marta G Castelhano, Brian W Davis, Lucy Davison, Ottmar Distl, Nicholas H Dodman, N Matthew Ellinwood, Jonathan E Fogle, Oliver P Forman, Dorian J Garrick, Edward I Ginns, Jens Häggström, Daisuke Hasegawa, Bianca Haase, Vidhya Jagannathan, Philippa Lait, Isabel Hernandez, Marjo K Hytönen, Maria Kaukonen, Tomoki Kosho, Emilie Leclerc, Teri L Lear, Tosso Leeb, Ronald H L Li, Hannes Lohi, Maria Longeri, Mark A Magnuson, Richard Malik, Shrinivasrao P Mane, Rondo Middleton, John S Munday, William J Murphy, Alexandra N Myers, Niels C Pedersen, Simon M Peterson-Jones, Max F Rothschild, Clare Rusbridge, Jeffrey J Schoenebeck, Beth Shapiro, Joshua A Stern, William F Swanson, Karen A Terio, Rory J Todhunter, Wesley C Warren, Elizabeth A Wilcox, Julia H Wildschutte, Yoshihiko Yu
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